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Mark Bloomberg writes to the sowacs list: ===8<============== My query relates to measuring moisture availability in the very small space around a germinating pine seed. This environment has a diameter ca 1cm, is close to the soil surface and therefore moisture levels fluctuate rapidly in relation to time of day, changes in atmospheric RH etc. Oleskog & Sahlen (Oleskog,G and Sahlen K. 1999. Determination of moisture conditions using Pinus sylvestris (L.) seeds as biosensors. Scandinavian journal of Forest Research.) stated that conventional methods of soil moisture measurement are not suitable because they may influence moisture levels in the seed's immediate environment, which is very small. There are also steep vertical gradients in moisture in the surface soil zone. Oleskog opted for a bioassay approach, using changes in seed fresh weight as an indicator of moisture availability. Does anyone have alternative views on this problem? Yours sincerely, Mark Mark Bloomberg Lecturer in Forestry SPES Division, Lincoln University Ph (64) (3) 325 2811 Ext 8437 ===8<============== NOTE: To get off this list, send an email to list@sowacs.com with the subject line: unsubscribe_sowacs For full instructions see this page: <http://www.sowacs.com/subscribe/index.html>